Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells
Identifieur interne : 001127 ( Main/Exploration ); précédent : 001126; suivant : 001128Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells
Auteurs : Mikako Takahoko [Japon] ; Minoru Tobiume [Japon] ; Koichi Ishikawa [Ghana] ; William Ampofo [Ghana] ; Naoki Yamamoto [Japon] ; Michiyuki Matsuda [Japon] ; Masashi Tatsumi [Japon]Source :
- AIDS research and human retroviruses [ 0889-2229 ] ; 2001.
Descripteurs français
- KwdFr :
- ADN recombiné (génétique), ADN recombiné (isolement et purification), Adulte, Agranulocytes (virologie), Analyse de séquence d'ADN, Cellules cancéreuses en culture, Clonage moléculaire, Données de séquences moléculaires, Humains, Infections à VIH (génétique), Infections à VIH (transmission), Infections à VIH (virologie), Mâle, Phylogénie, Réplication virale, Transfection, VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique).
- MESH :
- génétique : ADN recombiné, Infections à VIH, VIH-1 (Virus de l'Immunodéficience Humaine de type 1).
- isolement et purification : ADN recombiné.
- virologie : Adulte, Agranulocytes, Analyse de séquence d'ADN, Cellules cancéreuses en culture, Clonage moléculaire, Données de séquences moléculaires, Humains, Infections à VIH, Mâle, Phylogénie, Réplication virale, Transfection.
- Pascal (Inist)
- Wicri :
- geographic : Ghana.
English descriptors
- KwdEn :
- Adult, Blood, Blood cell, Breakpoint, CCR5 chemokine receptor, Cloning, Molecular, DNA, Recombinant (genetics), DNA, Recombinant (isolation & purification), Ghana, HIV Infections (genetics), HIV Infections (transmission), HIV Infections (virology), HIV-1 (genetics), HIV-1 virus, Humans, Infection, Isolate, Leukocytes, Mononuclear (virology), Male, Molecular Sequence Data, Phylogeny, Phytohemagglutinin, Recombination, Replication, Sequence Analysis, DNA, Transfection, Tumor Cells, Cultured, Use, Virus Replication.
- MESH :
- chemical , genetics : DNA, Recombinant.
- chemical , isolation & purification : DNA, Recombinant.
- genetics : HIV Infections, HIV-1.
- transmission : HIV Infections.
- virology : HIV Infections, Leukocytes, Mononuclear.
- Adult, Cloning, Molecular, Humans, Male, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Transfection, Tumor Cells, Cultured, Virus Replication.
Abstract
We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000134
- to stream PascalFrancis, to step Curation: 000014
- to stream PascalFrancis, to step Checkpoint: 000116
- to stream Main, to step Merge: 001200
- to stream PubMed, to step Corpus: 000661
- to stream PubMed, to step Curation: 000661
- to stream PubMed, to step Checkpoint: 000661
- to stream Ncbi, to step Merge: 000042
- to stream Ncbi, to step Curation: 000042
- to stream Ncbi, to step Checkpoint: 000042
- to stream Main, to step Merge: 001139
- to stream Main, to step Curation: 001127
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells</title><author><name sortKey="Takahoko, Mikako" sort="Takahoko, Mikako" uniqKey="Takahoko M" first="Mikako" last="Takahoko">Mikako Takahoko</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tobiume, Minoru" sort="Tobiume, Minoru" uniqKey="Tobiume M" first="Minoru" last="Tobiume">Minoru Tobiume</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ishikawa, Koichi" sort="Ishikawa, Koichi" uniqKey="Ishikawa K" first="Koichi" last="Ishikawa">Koichi Ishikawa</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Noguchi Memorial Institute for Medical Research, University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university">Université du Ghana</orgName></affiliation></author><author><name sortKey="Ampofo, William" sort="Ampofo, William" uniqKey="Ampofo W" first="William" last="Ampofo">William Ampofo</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Noguchi Memorial Institute for Medical Research, University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university">Université du Ghana</orgName></affiliation></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Microbiology, Tokyo Medical and Dental University</s1><s2>Tokyo 113-8519</s2><s3>JPN</s3><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsuda, Michiyuki" sort="Matsuda, Michiyuki" uniqKey="Matsuda M" first="Michiyuki" last="Matsuda">Michiyuki Matsuda</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tatsumi, Masashi" sort="Tatsumi, Masashi" uniqKey="Tatsumi M" first="Masashi" last="Tatsumi">Masashi Tatsumi</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Veterinary Science, National Institute of Infectious Diseases</s1><s2>Tokyo 162-8640</s2><s3>JPN</s3><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">02-0322075</idno><date when="2001">2001</date><idno type="stanalyst">PASCAL 02-0322075 INIST</idno><idno type="RBID">Pascal:02-0322075</idno><idno type="wicri:Area/PascalFrancis/Corpus">000134</idno><idno type="wicri:Area/PascalFrancis/Curation">000014</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000116</idno><idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000116</idno><idno type="wicri:doubleKey">0889-2229:2001:Takahoko M:infectious:dna:clone</idno><idno type="wicri:Area/Main/Merge">001200</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:11485626</idno><idno type="wicri:Area/PubMed/Corpus">000661</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000661</idno><idno type="wicri:Area/PubMed/Curation">000661</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000661</idno><idno type="wicri:Area/PubMed/Checkpoint">000661</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000661</idno><idno type="wicri:Area/Ncbi/Merge">000042</idno><idno type="wicri:Area/Ncbi/Curation">000042</idno><idno type="wicri:Area/Ncbi/Checkpoint">000042</idno><idno type="wicri:doubleKey">0889-2229:2001:Takahoko M:infectious:dna:clone</idno><idno type="wicri:Area/Main/Merge">001139</idno><idno type="wicri:Area/Main/Curation">001127</idno><idno type="wicri:Area/Main/Exploration">001127</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells</title><author><name sortKey="Takahoko, Mikako" sort="Takahoko, Mikako" uniqKey="Takahoko M" first="Mikako" last="Takahoko">Mikako Takahoko</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tobiume, Minoru" sort="Tobiume, Minoru" uniqKey="Tobiume M" first="Minoru" last="Tobiume">Minoru Tobiume</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Ishikawa, Koichi" sort="Ishikawa, Koichi" uniqKey="Ishikawa K" first="Koichi" last="Ishikawa">Koichi Ishikawa</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Noguchi Memorial Institute for Medical Research, University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university">Université du Ghana</orgName></affiliation></author><author><name sortKey="Ampofo, William" sort="Ampofo, William" uniqKey="Ampofo W" first="William" last="Ampofo">William Ampofo</name><affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Noguchi Memorial Institute for Medical Research, University of Ghana</s1><s2>Legon</s2><s3>GHA</s3><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Ghana</country><placeName><settlement type="city">Accra</settlement><settlement type="town">Legon (Ghana)</settlement><region type="region">Région du Grand Accra</region></placeName><orgName type="university">Université du Ghana</orgName></affiliation></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Department of Microbiology, Tokyo Medical and Dental University</s1><s2>Tokyo 113-8519</s2><s3>JPN</s3><sZ>5 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Matsuda, Michiyuki" sort="Matsuda, Michiyuki" uniqKey="Matsuda M" first="Michiyuki" last="Matsuda">Michiyuki Matsuda</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Pathology, Research Institute, International Medical Center of Japan</s1><s2>Tokyo 162-8644</s2><s3>JPN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>6 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Tatsumi, Masashi" sort="Tatsumi, Masashi" uniqKey="Tatsumi M" first="Masashi" last="Tatsumi">Masashi Tatsumi</name><affiliation wicri:level="1"><inist:fA14 i1="04"><s1>Department of Veterinary Science, National Institute of Infectious Diseases</s1><s2>Tokyo 162-8640</s2><s3>JPN</s3><sZ>7 aut.</sZ></inist:fA14><country>Japon</country><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></analytic><series><title level="j" type="main">AIDS research and human retroviruses</title><title level="j" type="abbreviated">AIDS res. hum. retroviruses</title><idno type="ISSN">0889-2229</idno><imprint><date when="2001">2001</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">AIDS research and human retroviruses</title><title level="j" type="abbreviated">AIDS res. hum. retroviruses</title><idno type="ISSN">0889-2229</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Blood</term><term>Blood cell</term><term>Breakpoint</term><term>CCR5 chemokine receptor</term><term>Cloning, Molecular</term><term>DNA, Recombinant (genetics)</term><term>DNA, Recombinant (isolation & purification)</term><term>Ghana</term><term>HIV Infections (genetics)</term><term>HIV Infections (transmission)</term><term>HIV Infections (virology)</term><term>HIV-1 (genetics)</term><term>HIV-1 virus</term><term>Humans</term><term>Infection</term><term>Isolate</term><term>Leukocytes, Mononuclear (virology)</term><term>Male</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Phytohemagglutinin</term><term>Recombination</term><term>Replication</term><term>Sequence Analysis, DNA</term><term>Transfection</term><term>Tumor Cells, Cultured</term><term>Use</term><term>Virus Replication</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN recombiné (génétique)</term><term>ADN recombiné (isolement et purification)</term><term>Adulte</term><term>Agranulocytes (virologie)</term><term>Analyse de séquence d'ADN</term><term>Cellules cancéreuses en culture</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Infections à VIH (génétique)</term><term>Infections à VIH (transmission)</term><term>Infections à VIH (virologie)</term><term>Mâle</term><term>Phylogénie</term><term>Réplication virale</term><term>Transfection</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1) (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Recombinant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Recombinant</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>HIV Infections</term><term>HIV-1</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN recombiné</term><term>Infections à VIH</term><term>VIH-1 (Virus de l'Immunodéficience Humaine de type 1)</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN recombiné</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>HIV Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Adulte</term><term>Agranulocytes</term><term>Analyse de séquence d'ADN</term><term>Cellules cancéreuses en culture</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Infections à VIH</term><term>Mâle</term><term>Phylogénie</term><term>Réplication virale</term><term>Transfection</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>HIV Infections</term><term>Leukocytes, Mononuclear</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Cloning, Molecular</term><term>Humans</term><term>Male</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Sequence Analysis, DNA</term><term>Transfection</term><term>Tumor Cells, Cultured</term><term>Virus Replication</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Virus HIV1</term><term>Cellule sanguine</term><term>Sang</term><term>Isolat</term><term>Ghana</term><term>Phylogenèse</term><term>Recombinaison</term><term>Point cassure</term><term>Transfection</term><term>Infection</term><term>Récepteur chimiokine CCR5</term><term>Utilisation</term><term>Phytohémagglutinine</term><term>Réplication</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Ghana</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We constructed an infectious DNA clone of the HIV-1 A/G recombinant 97GH-AG2, which was isolated in Ghana in 1997 and was classified originally as subtype A. By phylogenetic and recombination breakpoint analyses, p97GH-AG2 was grouped in the circulating form of A/G recombinants (CRF02―AG) and was found to contain the least amount of subtype G-derived region among the known CRF02―AG HIV-1 DNAs. This result suggests that CRF02―AG may be a predominant form in Ghana. Virions produced by transfection of p97GH-AG2 into 293T cells grew in phytohemagglutinin-stimulated peripheral blood mononuclear cells (PBMCs). 97GH-AG2 also replicated efficiently in CCR5-expressing HeLa cells, MAGICS, but only weakly in the parent MAGI cells, indicating that 97GH-AG2 uses mostly CCR5 as a coreceptor. Isolation of the first HIV-1 (CRF02―AG) DNA clone that replicates in PBMCs will accelerate the molecular analysis of this subtype.</div></front></TEI><affiliations><list><country><li>Ghana</li><li>Japon</li></country><region><li>Région de Kantō</li><li>Région du Grand Accra</li></region><settlement><li>Accra</li><li>Legon (Ghana)</li><li>Tokyo</li></settlement><orgName><li>Université du Ghana</li></orgName></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Takahoko, Mikako" sort="Takahoko, Mikako" uniqKey="Takahoko M" first="Mikako" last="Takahoko">Mikako Takahoko</name></region><name sortKey="Matsuda, Michiyuki" sort="Matsuda, Michiyuki" uniqKey="Matsuda M" first="Michiyuki" last="Matsuda">Michiyuki Matsuda</name><name sortKey="Tatsumi, Masashi" sort="Tatsumi, Masashi" uniqKey="Tatsumi M" first="Masashi" last="Tatsumi">Masashi Tatsumi</name><name sortKey="Tobiume, Minoru" sort="Tobiume, Minoru" uniqKey="Tobiume M" first="Minoru" last="Tobiume">Minoru Tobiume</name><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name></country><country name="Ghana"><region name="Région du Grand Accra"><name sortKey="Ishikawa, Koichi" sort="Ishikawa, Koichi" uniqKey="Ishikawa K" first="Koichi" last="Ishikawa">Koichi Ishikawa</name></region><name sortKey="Ampofo, William" sort="Ampofo, William" uniqKey="Ampofo W" first="William" last="Ampofo">William Ampofo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaGhanaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001127 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001127 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= SidaGhanaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:02-0322075
|texte= Infectious DNA clone of HIV type 1 A/G recombinant (CRF02―AG) replicable in peripheral blood mononuclear cells
}}
| This area was generated with Dilib version V0.6.31. |  |